| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Buchnera aphidicola | AGTTTCGTTATCGCCAATGCT | TCACGACGAAAACCTCCACT | 58.64 | 59.25 | 182 | 84 |
100.00%
|
25.00%
|
| Primer 2 | Buchnera aphidicola | AGTTTCGTTATCGCCAATGCT | TCACGACGAAAACCTCCACTT | 58.64 | 59.86 | 182 | 81 |
100.00%
|
25.00%
|
| Primer 3 | Buchnera aphidicola | AGTTTCGTTATCGCCAATGCT | TTCACGACGAAAACCTCCACT | 58.64 | 59.86 | 183 | 72 |
100.00%
|
25.00%
|
| Primer 4 | Buchnera aphidicola | AGTTTCGTTATCGCCAATGCT | TTCACGACGAAAACCTCCAC | 58.64 | 58.43 | 183 | 72 |
100.00%
|
25.00%
|
| Primer 5 | Buchnera aphidicola | AGTTTCGTTATCGCCAATGCT | CACGACGAAAACCTCCACTT | 58.64 | 58.42 | 181 | 69 |
100.00%
|
25.00%
|